C3a Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb101135
• Category: Antibodies
• Description: C3a Receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Equine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR (401-482/482aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: C3AR1
• UniProt ID: Q16581
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AZ 3B; AZ3B; C3a anaphylatoxin chemotactic recepto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

JM Koh, SA Moon, JM Kim, YS Lee, HJ Cho, YJ Choi, JH Yoon, D Kim, X Che, X VGF and VGF-derived peptide AQEE30 stimulate osteoblastic bone formation through C3a receptor researchsquare.com, (2024)

PubMed:

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (orb101135) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: K562 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Lung (Mouse) Lysate at 40 ug, Primary: Anti-C3a Receptor (orb101135) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 64 kD.