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Catalog Number | orb156039 |
---|---|
Category | Antibodies |
Description | C2orf72 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human C2orf72 (1-100/295aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 30 kDa |
Target | C2orf72 |
UniProt ID | A6NCS6 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | C2orf72; CB072_HUMAN; Chromosome 2 open reading fr Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C2orf72) Polyclonal Antibody, Unconjugated (orb156039) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HepG2 (human) cell Lysate at 40 ug, HL-60 (human) cell Lysate at 40 ug, Primary: Anti-C2orf72 (orb156039) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.
IHC-Fr, IHC-P, WB | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
HRP |
IHC-Fr, IHC-P, WB | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
AP |
IF | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Cy5 |
IF | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
PE/Cy5.5 |
IF | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
PE/Cy7 |