C22orf43 Rabbit Polyclonal Antibody

SKU: orb156020

Description

C22orf43 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human C22orf43 (1-100/229aa)
• Target: DRICH1
• Molecular Weight: 25 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

C22orf43; Chromosome 22 open reading frame 43; CV043_HUMAN; Putative uncharacterized protein C22orf43; Uncharacterized protein C22orf43.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C22orf43) Polyclonal Antibody, Unconjugated (orb156020) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C22orf43) Polyclonal Antibody, Unconjugated (orb156020) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HT29 (huaman) Cell Lysate at 40 ug, Primary: Anti-C22orf43 (orb156020) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25kD, Observed band size: 23kD.

Sample: U251 (human) Cell Lysate at 40 ug, Primary: Anti-C22orf43 (orb156020) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25kD, Observed band size: 22kD.

Quick Database Links

Gene Symbol

DRICH1

UniProt

Q6PGQ1

References

Mair, Barbara et al. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Rep, 27, 599-615.e12 (2019)

PubMed: 30970261