C21orf33 antibody

• Catalog Number: orb377957
• Category: Antibodies
• Description: Rabbit polyclonal antibody to C21orf33.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human KNP-I
• Dilution range: WB: 1:500-2000, IF/ICC: 1:10-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: C21orf33
• Entrez: 102724023, 8209
• UniProt ID: P30042
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HES1; KNPI; ES1 protein homolog mitochondrial; Pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KNP-I expression in SW480 (A), HepG2 (B), mouse skin (C), mouse heart (D), rat skeletal muscle (E) whole cell lysates. (Predicted band size: 24; 28 kD; Observed band size: 25 kD)

Immunofluorescent analysis of KNP-I staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.