C1QL1 Rabbit Polyclonal Antibody

• Catalog Number: orb1260
• Category: Antibodies
• Description: C1QL1 Rabbit Polyclonal Antibody
• Target: C1QL1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human C1QL1 (21-120/258aa)
• UniProt ID: O75973
• MW: 25 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C1q related factor; C1q-related factor; C1ql1; C1Q
• Research Area: Neuroscience
• Note: For research use only

References

Ningning Zhang # 1 2, Qing Shao # 1, Xinni Xiang 3, Chun Yan 4, Dan Tao 2 5, Qian Li 6, Huan Rong 2, Yi Zhao 2, Tingxiu Xiang 7, Xiaohua Zeng C1QL1 inhibits breast cancer through the HSP90α/VCP-ERS/UPR axis Exp Mol Med ., (2025)

PubMed: 40583061

Applications

IHC, WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C1QL1) Polyclonal Antibody, Unconjugated (orb1260) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-C1QL1 (orb1260) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 32 kD.

Tissue/Cell: mouse cerebel tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C1QL1 Polyclonal Antibody, Unconjugated (orb1260) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.