C17orf74 Rabbit Polyclonal Antibody

• Catalog Number: orb2636
• Category: Antibodies
• Description: C17orf74 Rabbit Polyclonal Antibody
• Target: SPEM2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human C17orf74 (401-501/501aa)
• UniProt ID: Q0P670
• MW: 57 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: chromosome 17 open reading frame 74; CQ074_HUMAN.
• Note: For research use only

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-C17orf74 (orb2636) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 63 kD.

Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C17orf74 Polyclonal Antibody, Unconjugated (orb2636) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C17orf74 Polyclonal Antibody, Unconjugated (orb2636) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.