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Catalog Number | orb1523550 |
---|---|
Category | Antibodies |
Description | c-Myb Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human, Mouse |
Immunogen | Between 1 and 50 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-c-Myb antibody A304-138A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | c-Myb |
UniProt ID | P10242 |
NCBI | NP_005366.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | proto-oncogene c-Myb; transcriptional activator My Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human c-Myb by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT-4 cells. Antibodies: Affinity purified rabbit anti-c-Myb antibody orb1523550 used for IP at 6 µg/mg lysate.
Detection of mouse c-Myb by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human c-Myb by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB
FC, ICC, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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