c-Myb Antibody

• Catalog Number: orb1523549
• Category: Antibodies
• Description: c-Myb Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-c-Myb antibody A304-138A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: c-Myb
• UniProt ID: P10242
• NCBI: NP_005366.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: proto-oncogene c-Myb; transcriptional activator My
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human c-Myb by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT-4 cells. Antibodies: Affinity purified rabbit anti-c-Myb antibody orb1523549 used for IP at 6 µg/mg lysate.

Detection of mouse c-Myb by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523549) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human c-Myb by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523549) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB