c-Kit Antibody

• Catalog Number: orb234304
• Category: Antibodies
• Description: c-Kit Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/mlWestern blot, 0.1-0.5μg/mlFlow Cytometry, 1-3μg/1x106cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 109865 MW
• UniProt ID: P10721
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mast/stem cell growth factor receptor Kit;SCFR;2.7
• Note: For research use only
• Application notes: WB: The detection limit for c-Kit is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-C-Kit antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of C-Kit using anti-C-Kit antibody.Lane 1:Recombinan Human C-Kit Protein 0.5ng.

WB analysis of C-Kit using anti-C-Kit antibody.Lane 1:HEPG2 Cell.

IHC analysis of C-Kit using anti-C-Kit antibody.C-Kit was detected in paraffin-embedded section of human intestinal cancer tissue.