Cart summary

You have no items in your shopping cart.

C-fos Rabbit Polyclonal Antibody

Catalog Number: orb10375

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10375
CategoryAntibodies
DescriptionC-fos Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human c-fos (1-100/380aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW41 kDa
TargetFOS
UniProt IDP01100
RRIDAB_10762854
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCellular oncogene fos; FBJ murine osteosarcoma vir
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
C-fos Rabbit Polyclonal Antibody

MCF-7 Cell (Human) Lysate at 30 ug, Embryo (Mouse) Lysate at 40 ug, RAW246.7 (Mouse) Lysate at 30 ug, NIH/3T3 (Mouse) Lysate at 30 ug, Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-c-fos (orb10375) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41kD, Observed band size: 55KD.

C-fos Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (orb10375) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

C-fos Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (orb10375) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

C-fos Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (orb10375) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

C-fos Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (orb10375) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

C-fos Rabbit Polyclonal Antibody

Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (orb10375) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

C-fos Rabbit Polyclonal Antibody

Tissue/Cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (orb10375) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

  • Phospho-SRF (Ser77) Rabbit Polyclonal Antibody [orb1497]

    IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Porcine

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • C-fos Rabbit Polyclonal Antibody [orb156348]

    IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Equine, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody [orb5857]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Canine, Gallus, Porcine, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • VEGF-D Polyclonal Antibody [orb1411834]

    IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • c-Fos Polyclonal Antibody [orb1414294]

    IF,  IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl