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Catalog Number | orb669759 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to c-erbB-2 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | FWP51 |
Tested applications | FC, IF, IP |
Reactivity | Human |
Isotype | IgG |
Immunogen | 20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | c-erbB-2 |
UniProt ID | P04626 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CD340; Receptor tyrosine-protein kinase erbB-2; Ne Read more... |
Note | For research use only |
Application notes | Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993). |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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