c-erbB-2 antibody

• Catalog Number: orb669758
• Category: Antibodies
• Description: Mouse monoclonal antibody to c-erbB-2
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FWP51
• Tested applications: FC, IF, IP
• Reactivity: Human
• Isotype: IgG1
• Immunogen: 20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: c-erbB-2
• UniProt ID: P04626
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD340; Receptor tyrosine-protein kinase erbB-2; Ne
• Note: For research use only
• Application notes: Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993).
• Expiration Date: 12 months from date of receipt.