BTN2A1 Rabbit Polyclonal Antibody

• Catalog Number: orb499606
• Category: Antibodies
• Description: BTN2A1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BTN2A1 (101-200/527aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: BTN2A1
• UniProt ID: Q7KYR7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: BK14H9.1; BT2.1; BT2A1_HUMAN; BTF1; BTN2A1; Butyro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yi Wang, Nan Ji, Yang Zhang, Junsheng Chu, Changcun Pan, Peng Zhang, Weiwei Ma, Xueguang Zhang, Jianzhong Jeff Xi, Mingze Chen, Yonghui Zhang, Liwei Zhang & Tao Sun B7H3-targeting chimeric antigen receptor modification enhances antitumor effect of V������������������9V������������������2 BMC, 672 (2023)

PubMed:

Reactivity

Human

Blank control: MCF7 cells (blue). Primary Antibody: Rabbit Anti-BTN2A1 antibody (orb499606), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: SH-Sy5Y. Primary Antibody (green line): Rabbit Anti-BTN2A1 antibody (orb499606), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BTN2A1) Polyclonal Antibody, Unconjugated (orb499606) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.