BTK antibody

• Catalog Number: orb338876
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BTK
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BTK. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BTK
• Entrez: 12229, 695
• UniProt ID: P35991, Q06187
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti AGMX1 antibody, anti ATK antibody, anti BPK a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BTK expression in Hela (A), mouse brain (B) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 75 kD)

Immunohistochemical analysis of BTK staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.