BTK Antibody

• Catalog Number: orb1273768
• Category: Antibodies
• Description: BTK Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7F12H4
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).
• Concentration: batch dependent
• Dilution range: Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
• Conjugation: Unconjugated
• Target: BTK
• UniProt ID: Q06187
• NCBI: Q06187
• Storage: BTK monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Ascitic fluid containing 0.03% sodium azide.
• Alternative names: AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinas
• Note: For research use only
• Application notes: Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
• Expiration Date: 12 months from date of receipt.

Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.

Immunofluorescense analysis of Jurkat cells using BTK monoclonal antibody with FITC-IgG (top). Immunohistochemical analysis of paraffin-embedded human lymph follicle tissue showing cytoplasmic and membrane location using BTK antibody with DAB (bottom).