BST2 Rabbit Polyclonal Antibody

SKU: orb1194

Description

BST2 Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, HIV, Immunology, Infectious Disease, Molecular Biology, NF-kB Signalling Pathway, Surface Molecules

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BST2/CD317 (1-100/180aa)
• Target: BST2
• Molecular Weight: 18 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Bone marrow stromal antigen 2; Bone marrow stromal cell antigen 2; Bone marrow stromal cell antigen; BST 2; BST-2; BST2_HUMAN; CD 317; CD317; CD317 antigen; HM1.24 antigen; NPC A 7; Tetherin.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-BST2 antibody (orb1194), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Lane 1: (Human/cell line) U937 lysates probed with BST2 Polyclonal Antibody, Unconjugated (Catalog #orb1194) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37°C.

Lane 1: (mouse/tissue) Lung lysates probed with BST2 Polyclonal Antibody, Unconjugated (Catalog #orb1194) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37°C.

Quick Database Links

Gene Symbol

BST2

UniProt

Q10589