BST2 Rabbit Polyclonal Antibody

• Catalog Number: orb1194
• Category: Antibodies
• Description: BST2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BST2/CD317 (1-100/180aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18 kDa
• Target: BST2
• UniProt ID: Q10589
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Bone marrow stromal antigen 2; Bone marrow stromal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-BST2 antibody (orb1194), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Lane 1: (Human/cell line) U937 lysates probed with BST2 Polyclonal Antibody, Unconjugated (Catalog #orb1194) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37°C.

Lane 1: (mouse/tissue) Lung lysates probed with BST2 Polyclonal Antibody, Unconjugated (Catalog #orb1194) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37°C.