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    Bromodeoxyuridine / BrdU Antibody

    Bromodeoxyuridine / BrdU Antibody

    Catalog Number: orb749407

    DispatchUsually dispatched within 5-10 working days
    $ 764.00
    Catalog Numberorb749407
    CategoryAntibodies
    DescriptionThis antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberBRD494
    Tested applicationsIHC-P
    ReactivityAll
    IsotypeMouse IgG1, kappa
    ImmunogenBrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
    Dilution rangeImmunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
    PurityProtein G affinity chromatography
    ConjugationUnconjugated
    Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
    Hazard InformationThis BrdU / Bromodeoxyuridine antibody is available for research use only.
    StorageStore the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
    Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
    NoteFor research use only
    Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Expiration Date12 months from date of receipt.
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