Bromodeoxyuridine / BrdU Antibody Cocktail

• Catalog Number: orb385716
• Category: Antibodies
• Description: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BRD469 + BRD494 + BRD.3
• Tested applications: IHC-P
• Reactivity: All
• Isotype: Mouse IgG1, kappa
• Immunogen: Bromodeoxyuridine was used as the immunogen for this BrdU antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BrdU antibody cocktail is available for research use only.
• UniProt ID: Not Applicable
• Storage: Store the BrdU antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the BrdU antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Mouse small intestine using BrdU Cocktail antibody