BrdU Antibody / Bromodeoxyuridine

• Catalog Number: orb699495
• Category: Antibodies
• Description: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BRDU/3902R
• Tested applications: IHC-P
• Reactivity: All
• Isotype: Rabbit IgG
• Immunogen: Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant BrdU antibody is available for research use only.
• UniProt ID: Not Applicable
• Storage: Store the recombinant BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.