BRD4 Antibody

• Catalog Number: orb1671549
• Category: Antibodies
• Description: BRD4 Antibody
• Clonality: Recombinant
• Clone Number: RAB-C131
• Tested applications: ChIP, ELISA, FC, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing BRD4 protein under non-denaturing conditions.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: BRD4
• UniProt ID: O60885
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: HUNK1, Bromodomain-containing protein 4, anti-BRD4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-BRD4 antibody R HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-BRD4 antibody R Nuclear lysate of K562 cells (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of fixed HeLa cells with anti-BRD4 antibody R. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671549- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.