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Catalog Number | orb1098040 |
---|---|
Category | Antibodies |
Description | BRD4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human BRD4 (QRPEMKPVDVGRPVIRPPEQ). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 200 kDa |
UniProt ID | O60885 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Expiration Date | 12 months from date of receipt. |
IF analysis of BRD4 using anti-BRD4 antibody. BRD4 was detected in an immunocytochemical section of PC-3 cells.
Western blot analysis of BRD4 using anti-BRD4 antibody.
Flow Cytometry analysis of C6 cells using anti-BRD4 antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.
Flow Cytometry analysis of U20S cells using anti-BRD4 antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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