BRD3 Rabbit Polyclonal Antibody

• Catalog Number: orb575083
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BRD3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BRD3
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: BRD3
• UniProt ID: Q15059
• Protein Sequence: Synthetic peptide located within the following region: SGKKQAAKSKEELAQEKKKELEKRLQDVSGQLSSSKKPARKEKPGSAPSG
• NCBI: NP_031397
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ORFX, RING3L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 2 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 61 kDa.

Sample Type: Human Adult Placenta, Antibody dilution: 1.0 ug/ml.

Sample Type: Human Fetal Lung, Antibody dilution: 1.0 ug/ml.

Positive control (+): Human lung (LU), Negative control (-): HeLa (HL), Antibody concentration: 1 ug/ml.

Hum. Adult Placenta.

Hum. Fetal Lung.

Immunohistochemistry with Testis tissue at an antibody concentration of 10 ug/ml using anti-BRD3 antibody (orb575083).

WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Human brain.