BRCA1 antibody

• Catalog Number: orb216035
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BRCA1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1524 of human BRCA1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BRCA1
• Entrez: 672, 497672, 12189
• UniProt ID: P38398, P48754, O54952
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti BRAC 1 antibody, anti BRAC-1 antibody, anti B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BRCA1 (Phospho-S1524) expression in U87MG (A), Myla2059 (B), A375 (C) whole cell lysates. (Predicted band size: 207 kD; Observed band size: 210 kD)

Immunohistochemical analysis of BRCA1 (Phospho-S1524) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.