Brain2 Rabbit Polyclonal Antibody

SKU: orb155867

Description

Brain2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Brain2/POU3F2 (301-400/443aa)
• Target: POU3F2
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Brain 2; Brain specific homeobox/POU domain protein 2; Brain-2; Brain-specific homeobox/POU domain protein 2; BRN 2; Brn-2; BRN2; N Oct 3; N OCT 3 Gene; Nervous system specific octamer binding transcription factor; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer Binding Transcription Factor 7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF 7; OTF-7; OTF7; PO3F2_HUMAN; POU class 3 homeobox 2; POU domain class 3 transcription factor 2; POU domain, class 3, transcription factor 2; Pou3f2; POUF 3; POUF3; Protein Brn 2.

Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Brain2 antibody (orb155867), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Primary: Anti-Brain2 (orb155867) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Brain2 (orb155867) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Quick Database Links

Gene Symbol

POU3F2

UniProt

P20265