BRAF antibody

• Catalog Number: orb153753
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BRAF. B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system. It has been observed that MAPK is activated by B-Raf in response to nerve growth factor. More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity. Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human B-Raf(NP_004324.2).
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Conjugation: Unconjugated
• MW: 85KDa
• UniProt ID: P15056
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti B-RAF1 antibody, anti BRAF1 antibody, anti NS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cells using BRAF antibody