B Raf/BRAF Antibody

• Catalog Number: orb215918
• Category: Antibodies
• Description: B Raf/BRAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 84437 MW
• UniProt ID: P15056
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine/threonine-protein kinase B-raf;2.7.11.1;Pro
• Note: For research use only
• Application notes: WB: The detection limit for B Raf is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-B Raf/BRAF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of B Raf/BRAF using anti-B Raf/BRAF antibody.Lane 1:human SH-SY5Y cell; 2:rat brain tissue; 3:rat testis tissue; 4:mouse brain tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of human differentiated adenocarcinoma of the rectum tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody. B Raf/BRAF was detected in a paraffin-embedded section of rat brain tissue.