BRAF Antibody

• Catalog Number: orb1240089
• Category: Antibodies
• Description: BRAF Antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgY
• Immunogen: Anti-B-raf antibody (orb1240089) was raised against a peptide corresponding to 18 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL.Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 94kDObserved: 94 kD
• Target: BRAF
• UniProt ID: P15056
• NCBI: P15056
• Storage: B-raf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-o
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1240089, (A: 1 µg/mL; B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1240089, (0.5 µg/mL), B-raf orb1239042, (0.5 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken (for orb1240089) and goat anti-rabbit (for orb1239042) IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1240089, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.