Cart summary

You have no items in your shopping cart.

Bovine Vimentin Protein

Bovine Vimentin Protein

Catalog Number: orb429207

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb429207
CategoryProteins
DescriptionBovine Vimentin protein
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 98.0% as determined by SDS-PAGE.
Solubility (25°C)It is recommended to reconstitute the Bovine Vimentin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SourceBovine Lens
StorageStability: Lyophilized Bovine Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BovineVimentin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Buffer/PreservativesThe protein (1mg/ml) was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate pH 7.5, 6M urea, 2mM DTT, 1mM EDTA and 10mM methylammonium chloride.
Alternative namesVimentin, Vim, FLJ36605.
Read more...
NoteFor research use only
Application notesReconstitution to filaments: After vimentin is dissolved in 6M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Sodium Phosphate, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco''s PBS).- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841.- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Expiration Date6 months from date of receipt.