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| Catalog Number | orb80023 |
|---|---|
| Category | Proteins |
| Description | Bovine FGF basic protein |
| Tested applications | SDS-PAGE |
| Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | Bovine FGF basic |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser. |
| Biological Origin | E.coli |
| Biological Activity | The ED50, measured in mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be < 0.1 ng/ml, corresponding to specific activity of 3,000,000 Units/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The FGF-b Bovine was lyophilized from concentrated (1mg/ml) sterile solution containing 1%HSA. |
| Alternative names | HBGH-2 protein, HBGF-2 protein, Prostatropin prote Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using calibrated solution of FGF-2 Bovine as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Bovine FGF basic protein
- Item 1 of 1
Bovine FGF2 Protein [orb1477238]
Greater than 90% as determined by SDS-PAGE.
18.4 kDa
Yeast
100 μg, 20 μg, 1 mg - Item 1 of 1
Bovine FGF2 Protein [orb1477360]
Greater than 90% as determined by SDS-PAGE.
20.5 kDa
E.coli
1 mg, 20 μg, 100 μg 
Recombinant Bovine Fibroblast Growth Factor-basic (rBoFGF-2) [orb1495071]
> 95% by SDS-PAGE and HPLC analyses.
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 172 amino acids
Escherichia coli.
1 mg, 100 μg, 10 μgFGF-2 Protein, Mouse, Recombinant [orb1962174]
97.10%
16 KDa (reducing condition)
1 mg, 500 μg, 10 μg, 50 μg
