Bovine FGF 2 Protein

• Catalog Number: orb425474
• Category: Proteins
• Description: Recombinant of bovine FGF 2 protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% HSA.
• Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser
• Application notes: Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard
• Source: Escherichia Coli
• Biological Activity: The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be < 0.1ng/ml, corresponding to a specific activity of 3 x 106 Units/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
• Alternative names: HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.
• Note: For research use only