Bone Alkaline Phosphatase Rabbit Polyclonal Antibody

• Catalog Number: orb100539
• Category: Antibodies
• Description: Bone Alkaline Phosphatase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bone Alkaline Phosphatase (56-150/524aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: ALPL
• UniProt ID: P05186
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AKP2; Alkaline phosphatase liver/bone/kidney; Alka
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

J Shiota, D Takahashi, L Chen, S Yokota, T Yutani Impact of different dosages of e-beam irradiation on mechanical strength of vitamin E-blended ultra-high-molecular-weight polyethylene and the osteolytic activities of its wear debris Materialia, (2025)

PubMed:

Applications

WB

Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4℃). Primary Antibody (green line): Rabbit Anti-Bone Alkaline Phosphatase antibody (orb100539), Dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.