BMP11 Rabbit Polyclonal Antibody

SKU: orb500856

Description

BMP11 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Differentiation, Neurogenesis, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Gallus, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BMP11 (301-407/407aa)
• Target: GDF11
• Molecular Weight: 12/43 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GDF11; BMP 11; Bone morphogenetic protein 11; GDF 11; Growth differentiation factor 11; Growth/differentiation factor 11; GDF11_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BMP11) Polyclonal Antibody, Unconjugated (orb500856) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-BMP11 (orb500856) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 12 kD, Observed band size: 12 kD.

Quick Database Links

Gene Symbol

GDF11

UniProt

O95390