BMP1 Antibody

• Catalog Number: orb1248101
• Category: Antibodies
• Description: BMP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-DTIVPKYEVNGVK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 110kDa
• Target: BMP1
• UniProt ID: P13497
• NCBI: NP_001190.1, NP_006120.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: BMP1, bone morphogenetic protein 1, OI13, PCOLC, P
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248101 (1 ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248101 (2 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248101 (2 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248101 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248101 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.