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BMP1 Antibody

SKU: orb1248101

Description

BMP1 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DTIVPKYEVNGVK
TargetBMP1
Molecular WeightApprox 110kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

BMP1, bone morphogenetic protein 1, OI13, PCOLC, PCP, PCP2, TLD, mammalian tolloid protein, procollagen C-endopeptidase, procollagen C-proteinase

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BMP1 Antibody

orb1248101 (1 ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

BMP1 Antibody

orb1248101 (2 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

BMP1 Antibody

orb1248101 (2 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

BMP1 Antibody

orb1248101 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

BMP1 Antibody

orb1248101 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

BMP1 Antibody (orb1248101)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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