BMI1 Antibody

• Catalog Number: orb1240095
• Category: Antibodies
• Description: BMI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1.The immunogen is located within amino acids 140 - 190 of BMI-1.
• Concentration: 1 mg/mL
• Dilution range: BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BMI1
• UniProt ID: P35226
• NCBI: P35226
• Storage: BMI-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, P
• Note: For research use only
• Application notes: BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/mL.

Immunocytochemistry of BMI-1 in K562 cells with BMI-1 antibody at 10 µg/mL.

Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 µg/mL.