BMI1 antibody

• Catalog Number: orb233612
• Category: Antibodies
• Description: Goat polyclonal antibody to BMI1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 36.9
• Target: BMI1 (aa237-251)
• Entrez: 648
• Protein Sequence: SHQRDGLTNAGELES
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti BMI1 antibody, anti BMI1 polycomb ring finger
• Note: For research use only
• Application notes: ELISA: antibody detection limit dilution 1:128000.WB: Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20(15):2110-20. PMID: 16882984). Recommended concentration: 0.1-0.3µg/ml. Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1 ). Recommended concentration: 1-3µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 nuclear lysate using BMI1 antibody

Western blot analysis of Pig Bone Marrow lysate using BMI1 antibody

Western blot analysis of NIH3T3 nuclear lysate using BMI1 antibody

Immunohistochemical staining of Human Testis using BM1 antibody.