Blood Group Lewis y Antibody

• Catalog Number: orb386050
• Category: Antibodies
• Description: This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A70-A/A9
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Lewis y antibody is available for research use only.
• UniProt ID: Not Applicable
• Storage: Store the Lewis y antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Lewis y antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human colon carcinoma using Blood Group Lewis y antibody