CD173 Antibody (PE)

• Catalog Number: orb1786025
• Category: Antibodies
• Description: CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
• Clonality: Monoclonal
• Clone Number: MEM-195
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgM
• Immunogen: Human thrombocytes
• Conjugation: PE
• Target: CD173
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Lewis Y, blood group H2 antigen, LeY
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD173 positive blood debris (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).