Blood Group Lewis a Antibody [SPM279]

• Catalog Number: orb1252404
• Category: Antibodies
• Description: Blood Group Lewis a Antibody [SPM279]
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Mucins isolated from ovarian cyst fluid were used as the immunogen for the Blood Group Lewis a antibody.
• UniProt ID: NotApplicable
• Tested applications: FC, IF, IHC-P
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Blood Group Lewis a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM279
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (SPM279).