BiP Rabbit Polyclonal Antibody

SKU: orb1294332

Description

Rabbit polyclonal antibody to BiP

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:2000-1:5000, Immunofluorescence 1:400-1:900, Immunohistochemistry (Paraffin) 1:100-1:300
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: BiP / GRP78
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-BiP / GRP78 antibody at 1/3000 dilution Lysate at 40 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294332 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. orb1294332 anti-Bip/ GRP78 antibody (red) at 1:500 and 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Bip/ GRP78 antibody with orb1294332 at 1/200.

Quick Database Links

Gene Symbol

BiP / GRP78