Biotin / Vitamin B7 / Vitamin H Antibody

• Catalog Number: orb2636593
• Category: Antibodies
• Description: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BTN/403
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: All
• Isotype: Mouse IgG1, kappa
• Immunogen: Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Biotin antibody is available for research use only.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Biotin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN/403). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.