Biotin / Vitamin B7 / Vitamin H Antibody

• Catalog Number: orb1151822
• Category: Antibodies
• Description: Mouse monoclonal antibody to Biotin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: All
• Immunogen: Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Application notes: Optimal dilution of the Biotin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: BTN399
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Biotin antibody is available for research use only.
• Note: For research use only

IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone BTN399) as confirmation of integrity and purity.