Biotin / Vitamin B7 / Vitamin H Antibody

• Catalog Number: orb1151822
• Category: Antibodies
• Description: Mouse monoclonal antibody to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BTN399
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: All
• Isotype: Mouse IgG1, kappa
• Immunogen: Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Biotin antibody is available for research use only.
• Storage: Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Biotin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone BTN399) as confirmation of integrity and purity.