BHMT antibody

• Catalog Number: orb19599
• Category: Antibodies
• Description: Goat polyclonal to BHMT
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 44.9
• Target: BHMT
• Entrez: 635
• Protein Sequence: EQQLKELFEKQK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti BHMT antibody, anti betaine-homocysteine meth
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.WB: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19599 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing BHMT RC203148) and probed with orb19599 (mock transfection in first lane), tested by Origene.

Immunohistochemical staining of Human Liver using BHMT antibody

Immunohistochemical staining of Human Kidney using BHMT antibody