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Catalog Number | orb666710 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to BF-1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic peptide of human BF-1. The exact sequence is proprietary. |
Dilution range | WB: 1-500-2000, IF: 1-50-200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | FOXG1 |
Entrez | 2290, 15228, 24370 |
UniProt ID | P55316, Q00939, Q60987 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-FKH2 antibody, anti-FKHL1 antibody, anti-FKHL Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of BF-1 expression in HEK293T (A), mouse brain (B), mouse kidney (C), rat spleen (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
Immunofluorescent analysis of BF-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
ELISA, FC, IF, IHC | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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