Beta galactosidase Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb462319
• Category: Antibodies
• Description: Beta galactosidase Rabbit Polyclonal Antibody (FITC)
• Target: GLB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Beta galactosidase (301-400/677aa)
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: BGAL_HUMAN; beta-galactosidase; GLB1; EC:3.2.1.23;
• Research Area: Angiogenesis, Apoptotic, Autophagic, Cancer, Cardi
• Note: For research use only

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-GLB1 antibody (orb462319), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.