APP Antibody

SKU: orb345773

Description

Beta Amyloid 42 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1: 10,000, WB: 1ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Beta Amyloid 1-42 antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Beta Amyloid 42 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of AB42.
• Target: APP
• Purity: Anti-Beta Amyloid 42 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with human, sheep, rabbit, canine, bovine, pig, guinea pig, and monkey based on 100% sequence homology. This antibody will not react with AB40. Cross-reactivity with Beta Amyloid from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Beta Amyloid 42 Antibody, ß-amyloid 42, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, Protease nexin-II

ELISA results of purified Polyclonal anti-beta amyloid 42 antibody tested against BSA-conjugated beta amyloid 42 peptide (green) and BSA-conjugated beta amyloid 40 peptide (blue). Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit IgG HRP conjugated (orb347654), and TMB substrate (p/n orb348657).

Quick Database Links

Gene Symbol

APP

UniProt

P05067

RefSeq (Protein):NP_000475.1