Beta Amyloid 31-35 Rabbit Polyclonal Antibody

• Catalog Number: orb499683
• Category: Antibodies
• Description: Beta Amyloid 31-35 Rabbit Polyclonal Antibody
• Target: APP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide of human beta-Amyloid (31-35/42aa)
• UniProt ID: P05067
• MW: 4.4 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: beta Amyloid(31-35); beta-Amyloid 31-35; A4; AAA;
• Research Area: DNA / RNA Binding, DNA and RNA Research, RNA Proce
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Mouse Spleen cells (blue). Primary Antibody (green line): Rabbit Anti-beta Amyloid 31-35 antibody (orb499683), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 0.1% Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.