Beta Amyloid 31-35 Rabbit Polyclonal Antibody

SKU: orb499683

Description

Beta Amyloid 31-35 Rabbit Polyclonal Antibody

Research Area

DNA / RNA Binding, DNA and RNA Research, RNA Processing

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg/Test
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide of human beta-Amyloid (31-35/42aa)
• Target: APP
• Molecular Weight: 4.4 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta Amyloid(31-35); beta-Amyloid 31-35; A4; AAA; ABETA; ABPP; AD1; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid beta A4 Protein Precursor; Amyloid beta; Amyloid of Aging and Alzheimer Disease; APP; APPI; B Amyloid; beta APP; Cerebral Vascular Amyloid Peptide; CTFgamma; CVAP; PN II; PN2; PreA4; Protease nexin II; A beta; Amyloid 31-35; A4_HUMAN.

Blank control (blue line): Mouse Spleen cells (blue). Primary Antibody (green line): Rabbit Anti-beta Amyloid 31-35 antibody (orb499683), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 0.1% Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

APP

UniProt

P05067