Beta Amyloid 1-42 Rabbit Polyclonal Antibody

SKU: orb10087

Description

Beta Amyloid 1-42 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide of human beta-Amyloid (1-42/42aa)
• Target: APP
• Molecular Weight: 4.4 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; alpha-sAPP; preA4; Adap; Ag; E030013M08Rik; betaApp; A4_HUMAN; APP; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); Protease nexin-II (PN-II); A4; A4_RAT; ABPP (APP); Amyloidogenic glycoprotein (AG);

Positive control: RSC 96 cells, Concentration: 1 µg/10^6 cells. Isotype control: Cell lines treated with rabbit IgG (orb570250) instead of the primary antibody to confirm that primary antibody binding is specific. Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (orb868805) to confirm no background signal produced from secondary antibody alone.

Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SY5Y cell lysates, Lane 3: Human U251 cell lysates, Primary: Anti-beta-Amyloid 1-40 (CT) (orb10087) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 4.3 kDa, Observed band size: 130 kDa.

Quick Database Links

Gene Symbol

APP

UniProt

P05067