Beta-Amyloid 1-40 (CT) Rabbit Polyclonal Antibody

SKU: orb10085

Description

Beta-Amyloid 1-40 (CT) Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human beta-Amyloid 1-40 (37-40/40aa)
• Target: APP
• Molecular Weight: 4.3 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; alpha-sAPP; preA4; Adap; Ag; E030013M08Rik; betaApp; A4_HUMAN; APP; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); Protease nexin-II (PN-II); A4; A4_RAT; ABPP (APP); Amyloidogenic glycoprotein (AG);

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta-Amyloid 1-40 (CT)) Polyclonal Antibody, Unconjugated (orb10085) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SY5Y cell lysates, Lane 3: Human U251 cell lysates, Primary: Anti-beta-Amyloid 1-40 (CT) (orb10085) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 4.3 kDa, Observed band size: 130 kDa.

Quick Database Links

Gene Symbol

APP

UniProt

P05067