Beta-Amyloid 1-40 (CT) Rabbit Polyclonal Antibody

• Catalog Number: orb10085
• Category: Antibodies
• Description: Beta-Amyloid 1-40 (CT) Rabbit Polyclonal Antibody
• Target: APP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human beta-Amyloid 1-40 (37-40/40aa)
• UniProt ID: P05067
• RRID: AB_10752628
• MW: 4.3 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: β-Amyloid 1-40; beta Amyloid(1-40); beta Amyloid 1
• Research Area: DNA / RNA Binding, DNA and RNA Research, RNA Proce
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta-Amyloid 1-40 (CT)) Polyclonal Antibody, Unconjugated (orb10085) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SY5Y cell lysates, Lane 3: Human U251 cell lysates, Primary: Anti-beta-Amyloid 1-40 (CT) (orb10085) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 4.3 kDa, Observed band size: 130 kDa.