Beta-Actin Rabbit Polyclonal Antibody (HRP)

SKU: orb110039

Description

Beta-Actin Rabbit Polyclonal Antibody (HRP)

Images & Validation

• Tested Applications: IHC-Fr, IHC-P, WB
• Dilution range: WB=1:5000-50000, IHC-P=1:100-500, IHC-F=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Feline, Fish, Gallus, Guinea pig, Hamster, Insect, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic MAP peptide derived from human beta-Actin (1-200/375aa)
• Target: ACTB
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; beta actin; beta cytoskeletal actin; A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Rat Uterus tissue lysates, Lane 4: Rat Rectum tissue lysates, Lane 5: Rat Placenta tissue lysates, Lane 6: Rat Lung tissue lysates, Lane 7: Rat Spleen tissue lysates, Lane 8: Human HL60 cell lysates, Lane 9: Human Huvec cell lysates, Lane 10: Human 293T cell lysates, Lane 11: Human Jurkat cell lysates, Lane 12: Human A549 cell lysates, Primary: Anti-beta-Actin/HRP (orb110039) at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Quick Database Links

Gene Symbol

ACTB

References

Tong, Yuna et al. The protective effect of shikonin on renal tubular epithelial cell injury induced by high glucose Biomed Pharmacother, 98, 701-708 (2018)

PubMed: 29304496