beta actin antibody

• Catalog Number: orb420637
• Category: Antibodies
• Description: Mouse monoclonal antibody to beta actin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8H10D10
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Dilution range: WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 41737
• Target: ACTB
• UniProt ID: P60709
• NCBI: NP_001092.1
• Alternative names: Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot:All lanes:Anti-Beta-actin Antibody at 1:2000 dilution + HL-60.

Western blot:All lanes:Anti-Beta-actin Antibody at 1:4000-1:8000 dilution.Lane 1:Hela;Lane 2:HepG2;Lane 3:A431;Lane 4:MCF-7;Lane 5:NIH/3T3.

Western blot analysis of HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody at 1:1000 dilution.A goat anti-mouse IgG H&L(HRP) was used as the secondary antibody.

Western blot analysis of HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody at 1:1000 dilution.

Immunohistochemical analysis of Human Ovarian cancer section using Pink1 at 1:50 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of H. spleen section using Beta-actin Antibody at 1:25 dilution.A peroxidase-conjugated goat anti-rabbit IgGwas used as the secondary antibody, followed by DAB staining.